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Effect of GUW treatment on <t>Evans</t> <t>blue</t> leakage after ischemia/reperfusion in rats. (A) Representative ex vivo NIRF imaging of the rats from different treatment group. At day 7 after MCAO, rats were infused with Evans blue <t>dye</t> and brains were harvested to evaluate the BBB integrity. (B) The Target-to-background ratios (TBRs) calculated from ROI analyses of NIRF image of Evans blue signal. The images were normalized on the color scaling bar. Data are expressed as mean ± SD. n=6. One-way ANOVA post hoc Tukey’s test. # p < 0.001 vs Sham group; * p < 0.001 vs MCAO.
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Effect of GUW treatment on Evans blue leakage after ischemia/reperfusion in rats. (A) Representative ex vivo NIRF imaging of the rats from different treatment group. At day 7 after MCAO, rats were infused with Evans blue dye and brains were harvested to evaluate the BBB integrity. (B) The Target-to-background ratios (TBRs) calculated from ROI analyses of NIRF image of Evans blue signal. The images were normalized on the color scaling bar. Data are expressed as mean ± SD. n=6. One-way ANOVA post hoc Tukey’s test. # p < 0.001 vs Sham group; * p < 0.001 vs MCAO.

Journal: bioRxiv

Article Title: Gastrodia-Uncaria Water Extract Inhibits Endoplasmic Reticulum Stress and Matrix Metalloproteinase in Protecting against Cerebral Ischemia

doi: 10.1101/2021.07.21.452303

Figure Lengend Snippet: Effect of GUW treatment on Evans blue leakage after ischemia/reperfusion in rats. (A) Representative ex vivo NIRF imaging of the rats from different treatment group. At day 7 after MCAO, rats were infused with Evans blue dye and brains were harvested to evaluate the BBB integrity. (B) The Target-to-background ratios (TBRs) calculated from ROI analyses of NIRF image of Evans blue signal. The images were normalized on the color scaling bar. Data are expressed as mean ± SD. n=6. One-way ANOVA post hoc Tukey’s test. # p < 0.001 vs Sham group; * p < 0.001 vs MCAO.

Article Snippet: At day 7 of post-operation, the animals were infused with 2 ml/kg of 2% Evans blue dye in saline (Sigma Aldrich, MO, USA) through the tail vein.

Techniques: Ex Vivo, Imaging